~
検索条件をクリア

アブストラクト

Japanese

Title SARS-CoV-2遺伝子検出法について
Subtitle 特集 COVID-19 第2波に備えてできること
Authors 白戸憲也
Authors (kana)
Organization 国立感染症研究所 ウイルス第3部
Journal 臨床とウイルス
Volume 48
Number 4
Page 248-257
Year/Month 2020 / 10
Article 報告
Publisher 日本臨床ウイルス学会
Abstract 〔論文要旨〕2019年12月末に中国武漢で発生した新型肺炎は, 新型コロナウイルス, すなわち重症呼吸器症候群コロナウイルス2 (SARS-CoV-2) によるものであった. 全長配列が公開され, すぐに遺伝子検査法が構築された. 感染研で構築されたリアルタイムRT-PCR法による検出法は全国地方衛生研究所, 検疫所に配布され, 行政検査に用いられた. 本稿では検査法開発の経緯とその後, およびリアルタイムRT-PCR法の特性について解説する.
Practice 臨床医学:内科系
Keywords COVID-19, SARSコロナウイルス, リアルタイムRT-PCR, SARS-CoV-2, Real-time RT-PCR

English

Title Development of genetic diagnostic methods for the detection of Severe Acute Respiratory Syndrome Coronavirus 2
Subtitle
Authors Kazuya SHIRATO
Authors (kana)
Organization Department of Virology III, National Institute of Infectious Diseases
Journal Clinical Virology
Volume 48
Number 4
Page 248-257
Year/Month 2020 / 10
Article Report
Publisher Japanese Society of Clinical Virology
Abstract At the end of December 2019, novel pneumonia emerged in Wuhan city, China, and it was caused by a novel coronavirus, i.e., Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The full-length sequence of SARS-CoV-2 was reported soon, and genetic diagnostic assays were developed immediately. The detection methods developed in the National Institute of Infectious Disease, Japan was distributed to prefectural and municipal public health institutes and quarantine depots for national surveillance tests. Here, we describe our genetic diagnostic methods, and the characteristics of real-time RT-PCR.
Practice Clinical internal medicine
Keywords COVID-19, SARS-CoV-2, Real-time RT-PCR
  • 全文ダウンロード: 従量制、基本料金制の方共に770円(税込) です。

参考文献

  • 1) Wu F, Zhao S, Yu B, et al.: A new coronavirus associated with human respiratory disease in China. Nature 2020 ; 579 : 265-269.
  • 2) Zhou P, Yang XL, Wang XG, et al.: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 2020 ; 579 : 270-273.
  • 3) Corman V, Eckerle I, Bleicker T, et al.: Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Euro Surveill 2012 ; 17 : 20285.
  • 4) Corman VM, Muller MA, Costabel U, et al.: Assays for laboratory confirmation of novel human corona-virus(hCoV-EMC) infections. Euro Surveill 2012 ; 17 : 20334.
  • 5) Corman VM, Landt O, Kaiser M, et al.: Detection of 2019 novel coronavirus(2019-nCoV) by real-time RT-PCR. Euro Surveill 2020 ; 25 : 2000045.
残りの16件を表示する
  • 6) Nao N, Shirato K, Matsuyama S, et al.: Detection of WN-Human1 sequence from clinical specimen. Available at : https://www.niid.go.jp/niid/images/vir3/nCoV/Method_NIID_20200114_Na.pdf
  • 7) Katano H, Suzuki T : Detection of 2019-novel coronavirus sequence from clinical specimen. Avail-able at : https://www.niid.go.jp/niid/images/pathol/pdf/Detection_of_nCoV_report200121.pdf
  • 8) Nao N, Shirato K, Katano H, et al.: Detection of second case of 2019-nCoV infection in Japan(cor-rected version). Available at : https://www.niid.go.jp/niid/images/vir3/nCoV/method-niid-20200123-2_erratum.pdf
  • 9) Shirato K, Nao N, Katano H, et al.: Development of genetic diagnostic methods for novel coronavirus 2019(nCoV-2019) in Japan. Jpn J Infect Dis 2020 ; doi : 10. 7883/yoken. JJID. 2020. 061.
  • 10) Okamoto K, Shirato K, Nao N, et al.: An assessment of real-time RT-PCR kits for SARS-CoV-2 detec-tion. Jpn J Infect Dis 2020 ; doi : 10. 7883/yoken. JJID. 2020. 108.
  • 11) 厚生労働省 : 臨床検査の保険適用について. Avail-able at : https://www.mhlw.go.jp/content/12404000/000558331.pdf
  • 12) 厚生労働省 : 新型コロナウイルス感染症の体外診断用医薬品(検査キット) の承認情報. Available at : https://www.mhlw.go.jp/stf/newpage_11331.html
  • 13) 厚生労働省健康局結核感染症課・国立感染症研究所 : 臨床検体を用いた評価結果が取得された2019-nCoV遺伝子検査方法について. Available at : https://www.niid.go.jp/niid/images/lab-manual/2019-nCoV-17-20200318.pdf
  • 14) Shirato K, Nao N, Matsuyama S, et al.: An ultra-rapid real-time RT-PCR method using the PCR1100 to detect Severe Acute Respiratory Syndrome Coronavirus-2. Jpn J Infect Dis 2020 ; 10. 7883/yoken. JJID. 2020. 324.
  • 15) Padhye NS : Reconstructed diagnostic serlsitivity and specificity of the RT-PCR test for COVID-19. medRxiv 2020 ; doi : https://doi.org/10.1101/2020.04.24.20078949.
  • 16) Williams E, Bond K, Zhang B, et al.: Saliva as a non-invasive specimen for detection of SARS-CoV-2. J Clin Microbiol 2020 ; 58(8): e00776-20.
  • 17) Iwasaki S, Fujisawa S, Nakakubo S, et al.: Compari-son of SARS-CoV-2 detection in nasopharyngeal swab and saliva. J Infect 2020 ; 81(2): e145-e147.
  • 18) Chau NVV, Lam VT, Dung NT et al.: The natural history and transmission potential of asymptomatic severe acute respiratory syndrome coronavirus 2 infection, Clin Infect Dis 2020 ; ciaa711, https://doi.org/10.1093/cid/ciaa711.
  • 19) Center for Disease Control and Prevention : In-terim guidelines for collecting, handling, and testing clinical specimens for COVID-19. Available at : https://www.cdc.gov/coronavirus/2019-ncov/lab/guidelines-clinical-specimens.html#specimen
  • 20) European Center for Diseases Prevention and Control : Laboratory support for COVID-19 in the EU/EEA. Available at : https://www.ecdc.europa.eu/en/novel-coronavirus/laboratory-support
  • 21) Public Health England : Guidance COVID-19 : laboratory investigations and sample requirements for diagnosis Available at : https://www.gov.uk/government/publications/wuhan-novel-coronavir-us-guidance-for-clinical-diagnostic-laborator-ies/laboratory-investigations-and-sample-requirements-for-diagnosing-and-monitoring-wn-cov-infection