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	Current Status of Development of Alternative Test Methods in Europe Tasuku Takamatsu Alternatives to Animal Testing and Experimentation 6(4): 129-135, 2000.

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	F-3. Significance of Experimental Animals in Safety Assessment of Chemicals Hiroshi Ono Alternatives to Animal Testing and Experimentation 6(4): 140-140, 2000.

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	F-4. Animal Experimentation is a Prerequisite for Biomedical Research Tsutomu Miki Kurosawa Alternatives to Animal Testing and Experimentation 6(4): 140-141, 2000.

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	F-5. Safety Evaluation and Animal Alternative Akihiro Kurishita Alternatives to Animal Testing and Experimentation 6(4): 141-141, 2000.

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	F-6. Concept of Animal Welfare and Research on the Alternative for Animal Experimentation Atsushige Sato Alternatives to Animal Testing and Experimentation 6(4): 141-142, 2000.

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	W-1 Is the Human Risk Assessment Possible with Animal Skin Toxicity Testing? Hajime Kojima Alternatives to Animal Testing and Experimentation 6(4): 142-142, 2000.

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	W-2. A New Serum-Free Medium, EpiLife(TM), Greatly Extends the Lifespan of Cultured Human Keratinocytes Making Large Banks of Highly-Characterized Normal Epithelial Cells Possible S.Li1, L.M.Donahue1, A.K.Shipley1, K.A.Droms1, Y.Lin1, G.D.Shipley1, M.Genno2, S.Niwata2, R.Yamamoto2 Alternatives to Animal Testing and Experimentation 6(4): 142-143, 2000.

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	W-3. Alternative Skin Irritation Test Methods Using Living Skin Equivalent Takuya Ishibashi1, Hidekazu Takahashi1, Mikio Nakagawa2, Keiichi Kawai2 Alternatives to Animal Testing and Experimentation 6(4): 143-143, 2000.

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	W-4. The Present Researches of Cultured Skin Model：Impression of Third World Congress on Alternatives and Animal Use in the Life Sciences Katsuyasu Morota Alternatives to Animal Testing and Experimentation 6(4): 143-143, 2000.

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	W-5. Present Situation of Skin Toxicity Evaluation in Europe Tasuku Takamatsu Alternatives to Animal Testing and Experimentation 6(4): 143-143, 2000.

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	W-6. Present Situation of Skin Toxicity Evaluation in US and in Japan Yasuo Ohno Alternatives to Animal Testing and Experimentation 6(4): 144-144, 2000.

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	PL. Conjugate-Dependent Toxicity：Alternatrves to Animal Studies M.W.Anders Alternatives to Animal Testing and Experimentation 6(4): 144-146, 2000.

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	AW-1. Validation Study on Five Cytotoxicity Assays by JSAAE II. Statistical Analysis T.Omori1, K.Saijo2, M.Kato2, M.Hayashi3, H.Itagaki4, S.Miyazaki5, T.Ohno2, H.Sugawara5, N.Teramoto2, N.Tanaka6, S.Wakuri6, I.Yoshimura1 Alternatives to Animal Testing and Experimentation 6(4): 146-146, 2000.

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	AW-2. Evaluation of Skin Irritation in a Reconstituted Human Dermal Model (3-D Model) Using Water Insoluble Fatty Acid, Fatty Alchols and Hydrocarbons Hajime Kojima, Atsushi Sato, Asao Hanamura, Tomonori Katada, Hiroaki Konishi Alternatives to Animal Testing and Experimentation 6(4): 146-147, 2000.

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	AW-3. Experience of 1st Validation Study on Five Different Cytotoxicity Assays Tadao Ohno Alternatives to Animal Testing and Experimentation 6(4): 147-147, 2000.

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	S-1. Framework of the Use of Human Materials in Pharmacogenetics：the Aim of This Symposium. Tohru Masui Alternatives to Animal Testing and Experimentation 6(4): 147-148, 2000.

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	S-2. Genetically Engineered Mammarian Cells, Bacteria And Teansgenic Animals Carrying Human Drug Metabolizing Enzymes：Perspcitives Tetsuya Kamataki Alternatives to Animal Testing and Experimentation 6(4): 148-148, 2000.

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	S-3. Functional Expression of Drug Metabolizing Enzymes in Novel Established Human Hepatoma Cell Lines and CDNA Epressed in Mammlian Cell Lines Masakiyo Hosokawa1, Hiroko Shinohara1, Yoko Tanaka1, Eiko Tsukada1, Mieko Mori1, Maki Fukumoto1, Yuko Ogasawara1, Seishi Nagamori2, Kan Chiba1 Alternatives to Animal Testing and Experimentation 6(4): 148-148, 2000.

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	S-4. High Throughput Monitoring of Hepatic Drug Metabolizing Enzymes Using Human Blood Tissues Shogo Ozawa Alternatives to Animal Testing and Experimentation 6(4): 149-150, 2000.

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	S-5. Ethical Considerations on the Use of Human Tissues and Cells Toshiharu Matsumura Alternatives to Animal Testing and Experimentation 6(4): 150-151, 2000.

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	S-6. Ethics of Human DNA Sampling Hiraku Takebe Alternatives to Animal Testing and Experimentation 6(4): 151-151, 2000.

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	S-7. Biosafety in Laboratories Working with Human Materials Reiko Hirai Alternatives to Animal Testing and Experimentation 6(4): 151-151, 2000.

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	S-8. Human Materials Resource in Japan：Present and Future Perspectives Tetsuo Satoh1, Satoshi Suzuki1, Norimitsu Kurata2, Yuki Noshimura2, Mariko Iwase2, Hua Li2, Youji Ito3, Mitsuo Kusano3, Yasuo Ohno4, Eiji Uchida2, Hajime Yasuhara2 Alternatives to Animal Testing and Experimentation 6(4): 152-152, 2000.

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	S-9. Public Research Resources：Human cell Hiroshi Mizusawa Alternatives to Animal Testing and Experimentation 6(4): 152-152, 2000.

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	P-1. Developmental Toxicity of 2,3,7,8-Tetrachlorodibenzo-p-Dioxin(TCDD) and Some of Its Relaed Compounds in Xenopus Laevis Shin Mima1, Michiko Sakamoto2, Takashi Tanrmura3 Alternatives to Animal Testing and Experimentation 6(4): 152-153, 2000.

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	P-2. Pharmacological and Toxicological Studies Using Chick Embryos (14) Diabetes Model in Chick Embryos Treated with Streptozotocin Takashi Sugiyama1, Miyuki Itoh1, Mariko Fukumoto2, Hideyo Shimada1,3, Kanji Tsuchimoto1,3. Alternatives to Animal Testing and Experimentation 6(4): 153-153, 2000.

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	P-3. In Vitro Embryotoxicity Testing of Metals for Dental Use by Differentiation of Embryonic Stem cells into Contracting Cardiac Myocytes Koichi Iman1, Horst Spielmann2, Gabriele Scholz2, Ingeborg Pohl2, Masaaki Nakamura1 Alternatives to Animal Testing and Experimentation 6(4): 153-154, 2000.

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	P-4. Evaluation of Embryotoxicity of Estradiol Benzoate and Diethylstilbestrol Using Whole Embryo Culture Makiko Kuwagata1, Chiaki Watanabe1, Hiromasa Takashima1, Hiroaki Aoyama2, Toshiaki Watanabe3, Hiroshi Ono1 Alternatives to Animal Testing and Experimentation 6(4): 154-154, 2000.

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	P-5. A New Differentiation Marker in Cultured Whole Embryos Neuronal Connections with Sensory Organs, in Special Reference to Vibrissa Rudiments Makoto Yamakado1, Masaharu Akita2, Atsushi Yokoyama2, Yukiaki Kuroda3 Alternatives to Animal Testing and Experimentation 6(4): 154-155, 2000.

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	P-6. The Histological Study of Whole Rat Embryos Cultured in Rat I.C. Serum Produced by Imamichi Institution of Animal Reproduction Masaharu Akita1, Atsushi Yokoyama1, Shigekazu Shimizu2, Hiroo Shikuma2, Yoshihiro Nozaki2, Yukiaki Kuroda3 Alternatives to Animal Testing and Experimentation 6(4): 155-155, 2000.

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	P-7. Effects of Trichlorobenzene as Endocrine Disrupting Chemical on Cultured Rat Embryos Atsushi Yokoyama1, Masaharu Akita1, Yukiaki Kuroda2 Alternatives to Animal Testing and Experimentation 6(4): 155-156, 2000.

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	P-8. Effects of Monochlorobenzene as an Endocrine Disrupting Chemical on Cultured Rat Embryos Atsushi Yokoyama1, Masaharu Akita1, Yukiaki Kuroda2 Alternatives to Animal Testing and Experimentation 6(4): 156-156, 2000.

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	P-9. Effects of Bisphenol A on Cultured Rat Embryos Masaharu Akita1, Atsushi Yokoyama1, Yukiaki Kuroda2 Alternatives to Animal Testing and Experimentation 6(4): 156-156, 2000.

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	P-10. Establishment of Downs Syndrome Cells to Study Cytotoxicity of Dental Materials Yoshitomo Ogawa1, Mikio Kato1, Daito Michiharu1, Koichi Imai2 Alternatives to Animal Testing and Experimentation 6(4): 157-157, 2000.

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	P-11. Inhibitory Effect of Enviromental Endocrine Disruptors on Thymosin α1 Production in Thymus Epithelial Cell Culture Kou Sakabe1, Takahiko Yoshida2, Hiroyuki Aikawa2, Shun-ichiro Izumi3, Yoshiyuki Osamura4, Fujio Kayama5 Alternatives to Animal Testing and Experimentation 6(4): 157-158, 2000.

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	P-12. Carcinogen Detection Potential of the BALB/3T3 Cell Taansformation Assay by Using a Medium Supplemented with Low Concentration of Serum and Some Growth Factors (Improved Assay Method) Yoshitsugu Kajiwara, Syozo Ajimi Alternatives to Animal Testing and Experimentation 6(4): 158-159, 2000.

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	P-13. Kinetic Analysis of Cell Killing Effect Induced by Busulfan in Chinese Hamster Cells Mariko Fukumoto1, Takashi Sugiyama2, Eriko Mitsuoka1, Akira Ogamo1, Kanji Tsuchimoto1 Alternatives to Animal Testing and Experimentation 6(4): 159-159, 2000.

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	P-14. Preparation and Evaluation of Artificial Membrane for Prediciting in Vitro Human Skin Absorption of Drugs Aki Matsuda1, Sachihiko Numajiri1, Daisuke Kobayashi1, Hideshi Natsume1, Masahiko Ogihara1, Yasunori Morimoto1, Ken Ichikawa2, Kurao Sudo2, Hiromi Kitano2 Alternatives to Animal Testing and Experimentation 6(4): 160-160, 2000.

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	P-15. Validation of the Neutral Red Uptake Phototoxicity Method Using Three Cell Lines Mariko Sugiyama, Hiroshi Itagaki, Yoshio Katsumura, Shinobu Kato Alternatives to Animal Testing and Experimentation 6(4): 160-160, 2000.

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	P-16. Evaluation of Cell Recovery Test Using a 3-Dimensional Human Cell Epidermis Model and Closed Patch Test for Skin Irritation Test Matsumi Terasawa, Noriko Nakagawa Alternatives to Animal Testing and Experimentation 6(4): 160-161, 2000.

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	P-17. Potential of LSE-High (Living Skin Equivlent) as a Model Membrane to Evaluate Skin Permeation and Skin Irritation of Drugs Tetsuya Watanabe1, Tetsuya Hasegawa1, Hidekazu Takahashi2, Takuya Ishibashi2, Masakazu Kiuchi3, Kozo Takayama1, Kenji Sugibayashi3 Alternatives to Animal Testing and Experimentation 6(4): 161-161, 2000.

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	P-18. Evaluation of Cosmetic Ingredients with Cultured Human Skin Model Tamie Suzuki, Masaki Uda, Itaru Miyamoto, Takao Ishida Alternatives to Animal Testing and Experimentation 6(4): 161-161, 2000.

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	P-19. In Vitro Systems for the Quantitative Predicton of Drug Interactions via the Hepatobiliary Transporers Yukio Kato1,2, Kanji Komatsu1, Kaoru Ueda1, Hiroshi Suzuki1,2, Yuichi Sugiyama1,2 Alternatives to Animal Testing and Experimentation 6(4): 162-162, 2000.

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	P-20. Cryopreserved Human Hepatocytes as a Tool for the prediction of in vivo Drug Transport and Transporter-Mediated Drug-Drug Interactions Yoshihisa Shitara1, Chuang Lu2, Albert P.Li2, Yukio Kato3, Hiroshi Suzuki3, Kiyomi Ito1, Tomoo Itoh1, Yuichi Sugiyama3 Alternatives to Animal Testing and Experimentation 6(4): 162-162, 2000.

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	P-21. Development of a simple Multi-Compartment Perfusion-Culture System for Toxicity Testing Including Absorption and Biotransformation Processes Motoyuki Suzuki, Terumasa Ohiso, Yasuyuki Sakai, Akiyoshi Sakoda Alternatives to Animal Testing and Experimentation 6(4): 162-163, 2000.

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	P-22. The Establishment and Characterizaton of Kidney-Derived Cell Lines Expressing Human Organic Anion Transporter 1 and 3 (hOAT1 and hOAT3) Shin-ichi Narikawa, Takashi Sekine, Seok Ho Cha, Makoto Hosoyamada, Michio Takeda, Hitoshi Endou Alternatives to Animal Testing and Experimentation 6(4): 163-163, 2000.

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