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P-1 Me-too validation study of reconstructed human corneal epithelial model, LabCyte CORNEA-MODEL24 eye irritation test method Saori HAGIWARA1), Shinsuke SHINODA1), Satoshi NAKAHARA2), Hajime KOJIMA3), Takashi OMORI4), Mai ENDO5), Maiko SATAKE5), Hidefumi IKEDA06), Hideki NISHIURA7), Toshihiko KASAHARA8), Yusuke YAMAMOTO8), Masakazu KATOH9), Katsura SUGAWARA9) Alternatives to Animal Testing and Experimentation 21(suppl): 120-120, 2016.

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	P-2 Study of the estimation scheme for developmental toxicity using in silico models and the toxicity mechanism based evaluations Junko OHUCHI, Hiroshi HONDA, Yuichi ITO, Osamu MORITA Alternatives to Animal Testing and Experimentation 21(suppl): 120-120, 2016.

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P-3 An alternative eye irritation test method for a three-dimensional reconstructed corneal model created using an immortalized human corneal epithelial cell line (iHCE-NY) : Performance in the prediction of eye irritants that incorporated the reversibility at the 30 reference chemicals of performance standards (TG492 PS) for reconstructed human cornea-like epithelium (Rh CE) testing. Yoshinao KATO1), Naoki YAMAMOTO2), Atsushi SATO1), Satoru NAKATA1), Hajime KOJIMA3) Alternatives to Animal Testing and Experimentation 21(suppl): 121-121, 2016.

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	P-4 Discriminant analysis of hepatotoxicity in rats by applying multiple statistical methods Jun-ichi TAKESHITA1), Haruka NAKAYAMA2), Yoko KITSUNAI2), Hitomi OKI2), Kouichi YOSHINARI2) Alternatives to Animal Testing and Experimentation 21(suppl): 121-121, 2016.

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	P-5 Attempt on the in silico prediction of rat hepatotoxicity using hierarchical clustering with molecular descriptors Kouichi YOSHINARI1), Haruka NAKAYAMA1), Yoko KITSUNAI1), Hitomi OKI1), Takuomi HOSAKA1), Takamitsu SASAKI1), Jun-ichi TAKESHITA2) Alternatives to Animal Testing and Experimentation 21(suppl): 122-122, 2016.

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	P-6 Development of an in situ evaluation system of ECM-modelized gel culture toward in vitro neurotoxicity tests Takayuki NAGAI1), Hideyuki MIZUMACHI1), Nana SHIRAKIGAWA2), Hiroyuki IJIMA2) Alternatives to Animal Testing and Experimentation 21(suppl): 122-122, 2016.

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	P-7 Biological profiling of chemical compounds based on the inhibition of cytochrome P450 activities Michiko WATANABE1), Yuki SHIMIZU1), Madoka KUSHIDA1), Hitomi OKI1), Jun-ichi TAKESHITA2), Takuomi HOSAKA1), Takamitsu SASAKI1), Kouichi YOSHINARI1) Alternatives to Animal Testing and Experimentation 21(suppl): 123-123, 2016.

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	P-8 Comparative metabolome analysis and toxicity test cultured fetal and adult hepatocytes in humans (3rd Report) Asuka SHIRAKI1), Yudai ICHIKAWA1), Kousuke INAMURA1), Takahiro MIZUTAMI1), Yuji KOMIZU1), Seiichi ISHIDA2), Su-Ryang KIM2), Yuko SEKINO2), Taku MATSUSHITA1) Alternatives to Animal Testing and Experimentation 21(suppl): 123-123, 2016.

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	P-9 Prediction of nanoparticles permeation through pulmonary alveolus using an in vitro co-culture model and a numerical simulation Ayaka UEMURA1), Xu XINYING1,2), Kikuo KOMORI1), Yasuyuki SAKAI1,2) Alternatives to Animal Testing and Experimentation 21(suppl): 124-124, 2016.

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	P-10 Development of new in vitro models for evaluation on the function of tight junction using CACO-2 cells Xintian LAO, Hiroko KOREISHI, Yasuyo DAININ, Tadayoshi UEDA Alternatives to Animal Testing and Experimentation 21(suppl): 124-124, 2016.

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	P-11 In vitro exposure study using a 3D cell culture model of human bronchial tissue for the assessment of aerosol chemicals Shinkichi ISHIKAWA, Shigeaki ITO Alternatives to Animal Testing and Experimentation 21(suppl): 125-125, 2016.

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	P-12 Enhancement of Th2 response by chemicals in food Taisei MIZOTA, Katsutoshi OHNO, Tetsuro OHBA Alternatives to Animal Testing and Experimentation 21(suppl): 125-125, 2016.

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	P-13 New 1h phenotypic screening allows efficacy and toxicity evaluation for anti-liver cancer against Chinese chive extract Toshihiro ONA1,2), Junko SHIBATA1,2), Teruyuki SEINO3) Alternatives to Animal Testing and Experimentation 21(suppl): 126-126, 2016.

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	P-14 Molecular clock in culture keratinocyte "Circadian mechanism of A QP3 expression" Naoya MATSUNAGA1,2), Kazufumi ITCHO2), Satoru KOYANAGI1,2), Shigehiro OHDO2) Alternatives to Animal Testing and Experimentation 21(suppl): 126-126, 2016.

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	P-15 Study of intracellular ROS production with photoreactivity Akemi TOYODA1), Kaoru KASAHARA1), Hiroshi ITAGAKI2) Alternatives to Animal Testing and Experimentation 21(suppl): 127-127, 2016.

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	P-16 Reducing false negative results in an in vitro skin sensitization test : The human cell line activation test Phuc Thi Hong VO1), Kazuto NARITA1), Fumiko NAKAGAWA1), Hajime KOJIMA2), Hiroshi ITAGAKI1) Alternatives to Animal Testing and Experimentation 21(suppl): 127-127, 2016.

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	P-17 Development of the effect of oxidative stress in the in vitro skin sensitization test Takafumi MITACHI, Minori MEZAKI, Hiroshi ITAGAKI Alternatives to Animal Testing and Experimentation 21(suppl): 128-128, 2016.

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	P-18 Development of an in vitro Skin Sensitization Test using differentiated THP-1 Kenta YAMAMOTO, Kazuto NARITA, Yuki ISHII, Hiroshi ITAGAKI Alternatives to Animal Testing and Experimentation 21(suppl): 128-128, 2016.

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	P-19 The effects of mixtures of chemicals on in vitro skin sensitization test Minori MEZAKI, Takafumi MITACHI, Mai KOUZUI, Hiroshi ITAGAKI Alternatives to Animal Testing and Experimentation 21(suppl): 129-129, 2016.

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	P-20 Development of a novel eye irritancy test using the Corneal Epithelium model Masayoshi HISAMA1), Sanae MATSUDA1), Norihiko ITO2,3) Alternatives to Animal Testing and Experimentation 21(suppl): 129-129, 2016.

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	P-21 Evaluation of skin sensitizer potency prediction model combining multiple in vitro tests Kaoru KASAHARA, Akemi TOYODA, Maki SUGIYAMA, Koichi NOMURA Alternatives to Animal Testing and Experimentation 21(suppl): 130-130, 2016.

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P-22 Reporting of training and transfer outcome for validation study of alternative method (ADRA) for skin sensitization using Cys and Lys derivatives Masaharu FUJITA1), Toshihiko KASAHARA1), Yusuke YAMAMOTO1), Shinichi WATANABE2), Tsunetsugu SUGAWARA2), Koji WAKABAYASHI3), Yu TAHARA3), Nobuyuki HORIE4), Keiichi FUJIMOTO4), Hiroaki TAKAHASHI5), Yoshihiko KUROKAWA5), Atsushi ONO6), Hajime KOJIMA6) Alternatives to Animal Testing and Experimentation 21(suppl): 130-130, 2016.

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	P-23 The feasibility of technology transfer of in vitro sensitization test, SH test (Part 2) Midori TAKEYOSHI1), Sakiko AIZAWA1), Noriyasu IMAI1), Maki SUGIYAMA2), Akemi TOYODA2) Alternatives to Animal Testing and Experimentation 21(suppl): 131-131, 2016.

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	P-24 Study of in vitro skin irritation test for ethanol-rich cosmetics Hidefumi IKEDA, Kinuko MIYAZAKI Alternatives to Animal Testing and Experimentation 21(suppl): 131-131, 2016.

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	P-25 Study of in vitro irritation testing in feminine hygene products using a reconstructed human vaginal mucosa epithelium model Shoko KOIZUMI, Hidefumi IKEDA, Masayuki TAKAISHI Alternatives to Animal Testing and Experimentation 21(suppl): 132-132, 2016.

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	P-26 The utility of the TRP channels to evaluate eye-stinging sensation Masayuki TAKAISHI1), Fumitaka FUJITA1), Shizuka IWASHITA1), Yuko SHIOMI1), Kunitoshi UCHIDA2), Yasunori TAKAYAMA2), Yoshiro SUZUKI2), Makoto TOMINAGA2) Alternatives to Animal Testing and Experimentation 21(suppl): 132-132, 2016.

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	P-27 An alternative test to estimate the potentials for phototoxic and photoallergic reactions Yuhma YOSHIDA1), Atsushi ODA2), Tatsuya HONDA1), Yuri OKANO2), Hitoshi MASAKI2) Alternatives to Animal Testing and Experimentation 21(suppl): 133-133, 2016.

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	P-28 The applicability of the alternative skin sensitization tests to plant extracts Taku NISHIJO, Kazutoshi SAITO, Yuki OTSUBO, Hideyuki MIZUMACHI, Masaaki MIYAZAWA, Hitoshi SAKAGUCHI Alternatives to Animal Testing and Experimentation 21(suppl): 133-133, 2016.

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P-29 Development of a skin sensitization test for poorly water-soluble substances using peptide-immobilized resins (2) : A modified direct peptide reactivity assay with a thiol or amino group indicators by spectrophotometry Hideaki MEKATA1), Yuuki MINAMINO2), Hiroshi MIYAZAKI3), Masayuki TAKAISHI1), Hidefumi IKEDA1), Kunihiko YAMASHITA3), Kenji USUI2) Alternatives to Animal Testing and Experimentation 21(suppl): 134-134, 2016.

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	P-30 Application of photo-KeratinoSens to Integrated Testing Strategy of photosensitization Hayato NISHIDA, Takao ASHIKAGA, Morihiko HIROTA, Hirokazu KOUZUKI Alternatives to Animal Testing and Experimentation 21(suppl): 134-134, 2016.

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	P-31 Development of an in vitro test for Allergenic Potency of Proteins Natsumi MATSUNARI1), Hanae TSUKUMO1), Xie DAN1), Akane OKA1), Hajime KOJIMA2), Hiroshi ITAGAKI1) Alternatives to Animal Testing and Experimentation 21(suppl): 135-135, 2016.

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	P-32 Development of non-animal assessment strategy for skin sensitization on a variety of raw materials Yuki OTSUBO, Hideyuki MIZUMACHI, Taku NISHIJO, Kazutoshi SAITO, Masaaki MIYAZAWA, Hitoshi SAKAGUCHI Alternatives to Animal Testing and Experimentation 21(suppl): 135-135, 2016.

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P-33 Development of a skin sensitization test for poorly water-soluble substances using peptide- immobilized resins (1) : A modified direct peptide reactivity assay with a peptide-immobilized resin containing hoto-labile linkers Yuuki MINAMINO1), Hiroshi MIYAZAKI2), Hideaki MEKATA3), Masayuki TAKAISHI3), Kunihiko YAMASHITA2), Kenji USUI1) Alternatives to Animal Testing and Experimentation 21(suppl): 136-136, 2016.

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	P-34 Development of evaluation system for skin care materials on sensitive skin using a reconstructed epidermal model Yukiko IZUTSU1), Yuki SATO1), Misaki HIRAYAMA2), Daiki KYOTANI3), Shoichi YAHAGI1), Hitoshi MASAKI2) Alternatives to Animal Testing and Experimentation 21(suppl): 136-136, 2016.

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	P-35 Safety assessment of Cosmetic Ingredients by Hand1-Luc EST Shiho OEDA, Akiko TAMURA, Takao ASHIKAGA, Hirokazu KOUZUKI Alternatives to Animal Testing and Experimentation 21(suppl): 137-137, 2016.

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	P-36 Usefulness of human iPS-derived cardiomyocytes in newly in vitro cardiotoxicity assay via multi-spheroid imaging analysis Tadashi NAGAKURA1), Takayoshi MATSUBARA2), Kohei SAWADA1) Alternatives to Animal Testing and Experimentation 21(suppl): 137-137, 2016.

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	P-37 Evaluation of drug-induced nephrotoxicity by cultured renal tubular epithelial cell line Yuya SAKAMOTO1), Takahisa YANO2), Aki TAKESHITA1), Fumika INAGAKI1), Naoya MATSUNAGA1,3), Satoru KOYANAGI1,3), Shigehiro OHDO1) Alternatives to Animal Testing and Experimentation 21(suppl): 138-138, 2016.

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	P-38 Mechanism-oriented integrated in vitro assay systems : Alternative methods of systemic toxicity test Shuichi SEKINE1), Moemi KAWAGUCHI1), Takumi NUKAGA1), Cong LIU1), Akinori TAKEMUR1), Takeshi SUSUKIDA1), Morihiko HIROTA2), Shiho OEDA2), Akiko TAMURA2), Takao ASHIKAGA2), Hirokazu KOUZUKI2), Kousei ITO1) Alternatives to Animal Testing and Experimentation 21(suppl): 138-138, 2016.

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	P-39 Development of drug permeability test utilizing corneal epithelium models fabricated in a collagen vitrigel membrane chamber : Effects of benzalkonium chloride on permeability of model drugs Hiroyuki YAMAGUCHI1,2), Toshiaki TAKEZAWA1) Alternatives to Animal Testing and Experimentation 21(suppl): 139-139, 2016.

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	P-40 Comparative assessment of in vitro hepatotoxicity tests based on mitochondrial toxicity Yuta MORIYAMA, Makoto MIYAUCHI, Kousuke MIYAZONO, Shigeki MATSUMOTO, Kazunari NAKAO Alternatives to Animal Testing and Experimentation 21(suppl): 139-139, 2016.

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	P-41 A novel co-culture system for extrapolating the excretion of hepatic metabolites into bile duct Ayumi Oshikata-MIYAZAKI, Toshiaki TAKEZAWA Alternatives to Animal Testing and Experimentation 21(suppl): 140-140, 2016.

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	P-42 An anti-cancer strategy targeting breast cancer stem cells : in vitro study using an exo-methylene compound and its possible application for cancer therapy Hiroyuki OKAZAKI1), Shuso TAKEDA2), Kenji MATSUMOTO3), Mitsuru SHINDO3), Hironori ARAMAKI1) Alternatives to Animal Testing and Experimentation 21(suppl): 140-140, 2016.

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	P-43 Expression of drug resistance of human hepatoma cells by Cellbed(R) 3D culture for screening of drug resistance overcoming reagent Maya NAKAMURA1), Yuya OHTA1), Takahiro MIZUTAM1), Yuji KOMIZU1), Takuya IWASA2), Kouhei SASAKI2), Rie WATANABE2), Masaaki KAWABE2), Taku MATSUSHITA1) Alternatives to Animal Testing and Experimentation 21(suppl): 141-141, 2016.

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	P-44 Evaluation in functional expression and maintenance culture of cryopreserved human hepatocyte using Cellbed(R) as 3D scaffold Hanaka NAGASAKI1), Kousuke INAMURA1), Takahiro MIZUTAMI1), Takaaki ISHII1), Yuji KOMIZU1), Takuya IWASA2), Kouhei SASAKI2), Rie WATANABE2), Masaaki WATANABE2), Taku MATSUSHITA1) Alternatives to Animal Testing and Experimentation 21(suppl): 141-141, 2016.

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	P-45 Protective effects of molecular hydrogen on hydrogen peroxide-induced oxidative stress in Hgc2 cardiomyocytes Hideto ARIUMI1), Hiroyuki MIYAZAKI2), Kohei INADA3), Hisamasa KODAIRA4), Yuji YOSHIYAMA1) Alternatives to Animal Testing and Experimentation 21(suppl): 142-142, 2016.

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	P-46 Inhibitory effect of Hydrogen gas on acetaminophen-induced hepatotoxicity in HepG2 cells Kana KOMATSU1), Yuji YOSHIYAMA1), Toshio KOZUKI2), Takeshi HONMA3), Shinji SUGIMOTO4), Takenori TAMAKI5), Hideto ARIUMI1) Alternatives to Animal Testing and Experimentation 21(suppl): 142-142, 2016.

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	P-47 Molecular clock-based analysis for mechanisms of VEGF induction in ischemic retina Naoki KUSUNOSE1), Yoshiyuki KOBAYASHI2), Shigeo YOSHIDA2), Kenichi KIMOTO1), Satoru KOYANAGI3), Shigehiro OHDO4), Toshiaki KUBOTA1) Alternatives to Animal Testing and Experimentation 21(suppl): 143-143, 2016.

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	P-48 Identification of the required amino acid for abnormal growth ability of oncogenetransformed cancer cells Shoya SHIROMIZU1), Yuta YAMAGUCHI1), Chiharu KATAMUNE1), Kenichi HASHIKAWA1), Chiho MURAKAMI1), Naoya MATSUNAGA1,2), Satoru KOYANAGI1,2), Shigehiro OHDO1) Alternatives to Animal Testing and Experimentation 21(suppl): 143-143, 2016.

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	P-49 Novel approach for treatment of triple negative breast cancer by focusing on transcriptional factor Takashi OGINO1), Naoya MATSUNAGA1,2), Yukinori HARA1), Takahiro TANAKA1), Satoru KOYANAGI1,2), Shigehiro OHDO1) Alternatives to Animal Testing and Experimentation 21(suppl): 144-144, 2016.

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	P-50 Exploring novel chemical compound preventing the onset of hepatocellular carcinoma Kazuhiro HIGASHI1), Akito TSURUTA1), Miyako WATANABE11), Naoya MATSUNAGA1,2), Keisuke KAKIMOTO1), Satoru KOYANAGI1,2), Shigehiro OHDO1) Alternatives to Animal Testing and Experimentation 21(suppl): 144-144, 2016.

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	P-51 Different roles of negative and positive components of the circadian clock in oncogene-induced neoplastic transformation in vitro Chiharu KATAMUNE1), Shoya SHIROMIZU1), Ken-ichi HASHIKAWA1), Naoya MATSUNAGA2), Satoru KOYANAGI2), Shigehiro OHDO1) Alternatives to Animal Testing and Experimentation 21(suppl): 145-145, 2016.

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	P-52 Trial of alternatives to animal experiments by hybrid gas permeable 3 dimensional cell culture plate Makoto KODAMA1), Yuki KINOSHITA1), Takehiko AOKI2), Yoshinori KANAHAMA2) Alternatives to Animal Testing and Experimentation 21(suppl): 145-145, 2016.

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	P-53 Adhesion and cytokine production of THP-1 cells cultured on an improved type of collagen vitrigel membrane chamber Tadashi UCHINO1), Hiroshi MIYAZAKI2), Kunihiko YAMASHITA3), Toshiaki TAKEZAWA4), Hajime KOJIMA1), Takumi AKIYAMA1), Yoshiaki IKARASHI11) Alternatives to Animal Testing and Experimentation 21(suppl): 146-146, 2016.

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P-54 Establishment of in vitro culture system mimicking organisms by co-culture of hepatic progenitor cell HepaRG and stellate cell Seiichi ISHIDA1), Shinichiro HORIUCHI1), Mitsuko HARA2), Yukie KURODA1), Shoko UCHIDA1), Riho ISHIDA1), Su-Ryang KIM1), Tomokazu MATSUURA3), Fabrice MOREL4), Anne CORLU4), Soichi KOJIMA2), Yuko SEKINO1) Alternatives to Animal Testing and Experimentation 21(suppl): 146-146, 2016.

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	P-55 Human skin model irritation test using three-dimensional fluorescence imaging Ryutaro AKIYOSHI Alternatives to Animal Testing and Experimentation 21(suppl): 147-147, 2016.

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	P-56 Analysis of the gene expression in response to DNA damage in plant Shinya NANAUMI, Kazuyuki HIRATSUKA, Shin-ichi OGATA Alternatives to Animal Testing and Experimentation 21(suppl): 147-147, 2016.

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	P-57 Gene expression and signaling pathway analysis of PTCH1 in cancer and stem cells Shihori TANABE1), Kazuhiko AOYAGI2), Takeshi KAWABATA3), Tetsuo YAMAGUCHI4), Hiroshi YOKOZAKI5), Hiroki SASAKI2) Alternatives to Animal Testing and Experimentation 21(suppl): 148-148, 2016.

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	P-58 Evaluation of Individual Exosomes Released from Human Breast Cancer SkBr3 Cells using Takanori AKAGI1), Hiromi KISHITA1), Yoko SUEHIRO1), Takanori ICHIKI1,2) Alternatives to Animal Testing and Experimentation 21(suppl): 148-148, 2016.

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	P-59 Identification of microRNA regulating OATP1B1 expression in human liver Yu EGASHIRA, Takeshi HIROTA, Sasagu KAWANO, Saki HIGASHIHARA, Ichiro IEIRI Alternatives to Animal Testing and Experimentation 21(suppl): 149-149, 2016.

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	P-60 Association between DNA methylation in the 5' CpG island of SLC47A1 (MATE1) gene and the inter-individual variability in MATE1 expression in the human liver Toshihiro TANAKA, Takeshi HIROTA, Ichiro IEIRI Alternatives to Animal Testing and Experimentation 21(suppl): 149-149, 2016.

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	P-61 Single nucleotide polymorphisms in the human PCFT promoter alter transcriptional activity in human intestine Takeshi HIROTA1), Shogo MIKI1), Toshio KUMAI2), Katsuhisa INOUE3), Hiroaki YUASA4), Ichiro IEIRI1) Alternatives to Animal Testing and Experimentation 21(suppl): 150-150, 2016.

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	P-62 Effect of oxygen condition on the differentiation of embryoid bodies of iPS cells Daisuke MIYAMOTO, Kohji NAKAZAWA Alternatives to Animal Testing and Experimentation 21(suppl): 150-150, 2016.

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	P-63 Basic studies of cytotoxicity assays by using plant cultured cells Riko ITO1), Hiroshi ITAGAKI2), Kazuyuki HIRATSUKA1), Shin-ichi OGATA1) Alternatives to Animal Testing and Experimentation 21(suppl): 151-151, 2016.

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	P-64 The effect on cellular function and microstructures of hepatic spheroids by using a novel method for loading ECM thin layer into spheroids Fumiya TAO1), Shigehisa AOKI2), Nobuhiko KOJIMA1) Alternatives to Animal Testing and Experimentation 21(suppl): 151-151, 2016.

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	P-65 Inhibition of necrosis in multicellular spheroids by improving tissue structures and culture environments Hirotaka MIHARA1), Yasuyuki SAKAI2), Nobuhiko KOJIMA1) Alternatives to Animal Testing and Experimentation 21(suppl): 152-152, 2016.

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	P-66 Study of scaffolds of withstanding the long-term differentiation culture of ES cells - String-like processed new tilapia scales derived collagen sponge - Koichi IMAI1), Tsubasa SHIRAI1), Hiroki ISHIKAWA2), Tamaki NAKANISHI2) Alternatives to Animal Testing and Experimentation 21(suppl): 152-152, 2016.

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	P-67 Feasibility of human hepatocytes culture using oxygen-permeable membranes Keiichi KIMURA1), Marie SHINOHARA1), Wenjin XIAO1), Yasuyuki SAKAI1,2) Alternatives to Animal Testing and Experimentation 21(suppl): 153-153, 2016.

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	P-68 Student assessment of the 3Rs and attempt to develop alternative methods in an animal experiment class of Wayo Women's University Ayane SATO, Mai IIDA, Toshikazu SUZUKI Alternatives to Animal Testing and Experimentation 21(suppl): 153-153, 2016.

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	P-70 Advanced in vitro toxicity test using the area of embryoid body Song Ai KANG, Hee Young KANG, Jin Yong AN, Eui-Bae JEUNG Alternatives to Animal Testing and Experimentation 21(suppl): 154-154, 2016.

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	P-71 EpiSkin Micronucleus Assay : a 3D approach in the assessment of the genotoxicity of ingredients Nan LI1), Lizao CHEN1), Yanfeng LIU1), Lingyan ZHONG1), Brigitte FAQUET2), Gladys OUEDRAOGO2) Alternatives to Animal Testing and Experimentation 21(suppl): 154-154, 2016.

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P-72 In vitro to in vivo extrapolation of hepatic metabolism in fish : an interlaboratory comparison of in vitro methods Kellie FAY1), Michelle EMBRY2), Mary Jo BERNHARD3), Ina BISCHOF4), John DAVIS5), Jean DOMORADZKI6), Marlies HALDER7), Jing HU5), Karla JOHANNING8), Heike LAUE9), Diane NABB10), John NICHOLS1), Heather PETERSON10), Christian SCHLECHTRIEM4), Helmut SEGNER111), John WEEKS12) Alternatives to Animal Testing and Experimentation 21(suppl): 155-155, 2016.

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	P-73 The Lush Prize Young Researcher Asia Awards 2016 Rebecca RAM Alternatives to Animal Testing and Experimentation 21(suppl): 155-155, 2016.

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	P-74 PAL-12, a new hexa-peptoid, can attenuate photo-aging effects in human dermal fibroblasts and 3D reconstructed human full skin model Da-Eun SONG, Hyeonji PARK, Kyung-Min LIM Alternatives to Animal Testing and Experimentation 21(suppl): 156-156, 2016.

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	P-75 Evaluating the efficacy of UV filters in sunscreen using 3D reconstructed skin model Jungah KIM1), Kyung-Mi JOO2), Kyung-Min LIM1) Alternatives to Animal Testing and Experimentation 21(suppl): 156-156, 2016.

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P-76 Evaluation of skin irritation and sensitization of hair dye components, Resorcinol, 2-Amino-5-Nitrophenol 2-Nitro-p-Phenylenediamine and p-phenlyenediamine, and comparison between two commercial hair dye products using 3-Dimensional Reconstructed Human Epidermis Model, Keraskin(TM) Hanseul PARK1), Kyung-Mi JOO2), Kyung-Min LIM1) Alternatives to Animal Testing and Experimentation 21(suppl): 157-157, 2016.

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	P-77 Inhibitory effect of 4-hydroxysattabacin on melanogenesis in 2D murine B16F10 melanoma cells and pigmented artificial human skin model, Melaskin(TM) Kyuri KIM, Sangjip NAM, Kyung-Min LIM Alternatives to Animal Testing and Experimentation 21(suppl): 157-157, 2016.

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	P-78 Serious eye damage / eye irritation assessment : SkinEthic - HCE reconstructed human corneal test method relevance and reliability after Asia Pacific region shipment Mariko YOKOTA1), Virginie LEBLANC2), Yuki SATO1), Daisuke YOSHIDA1), Daiki KYOTANI3), Nathalie ALEPEE2), Jose COTOVIO2) Alternatives to Animal Testing and Experimentation 21(suppl): 158-158, 2016.

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	P-79 Evaluation of toxicants for the neural differentiation of human ESCs Jin Yong AN, Hee Young KANG, Song Ai KANG, Eui-Bae JEUNG Alternatives to Animal Testing and Experimentation 21(suppl): 158-158, 2016.

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	P-80 Development the technique for the Preparation of the 3D Reconstructed Human Epidermis (RHE) Herlina B.SETIJANTI, Eka RUSMAWATI, Rahmi FITRIA, Tuty ERLINA, Rina ADRIANY, MURTININGSIH Alternatives to Animal Testing and Experimentation 21(suppl): 159-159, 2016.

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	P-81 Transcriptional profiling study of cosmetic preservatives on the discovery of novel biomarkers for a novel skin irritation tests Jungmin KIM1), Sae On KIM1), Sun Hee JIN1), Soo Hyun HONG1), Minsoo NOH1,2) Alternatives to Animal Testing and Experimentation 21(suppl): 159-159, 2016.

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	P-82 Preliminary evaluation of vascular endothelial growth factor as a biomarker for alternative skin sensitization test in HaCaT keratinocytes Sae On KIM1), Jungmin KIM1), Sun Hee JIN1), Soo Hyun HONG1), Minsoo NOH1,2) Alternatives to Animal Testing and Experimentation 21(suppl): 160-160, 2016.

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	P-83 An integrated framework for SAR-based read-across in P&G Sanae TAKEUCHI1), Ying WANG2), Seok KWON3), Shengde WU4), Sharon STUARD4), George DASTON4), Karen BLACKBURN4) Alternatives to Animal Testing and Experimentation 21(suppl): 160-160, 2016.

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	IAR1 Use of oxygen-permeable plates as a new systems for liver cell culture Yasuyuki SAKAI1,2), Wenjin XIAO2), Keiichi KIMURA2), Marie SHINOHARA2), Kikuo KOMORI1) Alternatives to Animal Testing and Experimentation 21(suppl): 161-161, 2016.

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	IAR2 Asian Advancement of Alternatives to Animal Experiment (3Rs) Tsutomu Miki KUROSAWA Alternatives to Animal Testing and Experimentation 21(suppl): 161-161, 2016.

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